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KMID : 0385520090220020148
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Analytical Science & Technology
2009 Volume.22 No. 2 p.148 ~ p.151
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Enantiomer separation using a covalently immobilized chiral column derived from polysaccharide derivative by reversed phase liquid chromatography
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Huang Hu
Jin Jing-Yu
Baek Chae-Sun
Lee Won-Jae
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Abstract
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Enantiomer separation of N-fluorenylmethoxycarbonyl (FMOC) α-amino acid was performed on covalently immobilized chiral column (Chiralpak IB) based on polysaccharide derivative as a chiral selector by reversed phase liquid chromatography. The effect of the reversed mobile phase on the chromatographic parameters of the enantioselectivities, resolution factors and retention times using covalently immobilized Chiralpak IB was shown. Also the enantiomer separation of N-FMOC α-amino acid in the reversed and normal phase was compared and the results obtained in the former mobile phase were generally lower than those in the latter mobile phase.
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KEYWORD
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enantiomer separation, chiral column, amino acid derivative, liquid chromatography
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